Hematoxylin and Eosin (H&E) staining Hematoxylin and Eosin (H&E) staining is histology’s most widely used method. It highlights cellular and tissue structure, enabling the identification of nuclei, cytoplasm, and extracellular Read More …
Author: Ms. Sangeeta Gourh
Practical section cutting
The success of microtome sectioning depends on both technique and equipment. Below is an in-depth look at practical section cutting, common cutting faults, and how to address them to achieve Read More …
Microtome knives and knife sharpening
Introduction Microtome knives and knife sharpening are essential in laboratories, especially in histology and materials science, for preparing ultra-thin specimen sections suitable for microscopic examination. These knives must maintain sharp Read More …
Microtome
Microtomes are essential instruments in laboratories for cutting ultra-thin slices of specimens, particularly for microscopy. Different types are suited for various materials and methods. Here’s a breakdown of types, working Read More …
Decalcification
Decalcification is a critical preparatory step in histology, particularly for hard tissues such as bone and teeth, which contain large amounts of calcium that make them challenging to section. Proper Read More …