Practical section cutting
Introduction Practical section cutting is one of the most important technical procedures in histopathology because the final microscopic diagnosis depends […]
Quality Control in Histopathology
Introduction Quality control in histopathology means a systematic process of monitoring all laboratory activities to ensure that tissue diagnosis is
Internal Quality Control and External Quality Assessment of Immunohistochemistry
Introduction Immunohistochemistry is one of the most important diagnostic techniques used in modern pathology for identification of specific cellular antigens
Microtome knives and knife sharpening
Introduction Microtome knives and knife sharpening are essential in laboratories, especially in histology and materials science, for preparing ultra-thin specimen
Lipid identification and demonstration
Introduction Lipids are essential biomolecules that play a critical role in cellular structure, energy storage, and signaling functions. They are
Male Reproductive System
Introduction The male reproductive system consists of specialized organs that are responsible for the production, maturation, and transport of male
Urinary System
Introduction Urinary system consists of: A pair of kidneys A pair of ureters Urinary bladder Urethra Kidneys produce urine. Urine
Handling fresh histological specimens
Introduction Handling of fresh histological specimens is the first and most critical step in the histopathology workflow. The quality of
Hematoxylin and Eosin Staining
Introduction Hematoxylin and Eosin staining (H&E) is the most commonly used routine staining technique in histopathology. It is the first-line
Labeling of histology specimens
Introduction Labeling of histology specimens is a crucial pre-analytical step in histopathology and diagnostic laboratories. It involves accurate identification and
Microtome
Introduction Microtomes are precision laboratory instruments used for cutting uniform, ultra-thin sections of specimens. They are essential in histology, pathology,
Histology laboratory equipment
Introduction Histology laboratory equipment is essential for preparing and examining tissue samples for microscopic study. These tools ensure proper fixation,
Histopathology lab safety
Introduction Histopathology involves processing biological tissues using various chemical, mechanical, and heat-based procedures. Lab personnel are exposed to: Infectious agents
Preparation of Tissue Sections in Histopathology
Introduction The preparation of tissue sections is an essential step in histopathology. Different techniques are used depending on whether we
Methods of Examination of Tissues and Cells
Introduction Histopathology = study of tissues under the microscope to detect disease.Cytopathology = study of cells under the microscope for
Processing histological tissues for paraffin embedding
Introduction Processing histological tissues for paraffin embedding is a fundamental technique in histopathology and biomedical research. This process is essential
Gross Examination of Histopathology
AIM: Gross Examination of Histopathological Specimens Principle The principle of gross examination of histopathology involves the visual and tactile assessment
Decalcification
Introduction Decalcification is a critical preparatory step in histology, particularly for hard tissues such as bone and teeth, which contain
Fixatives
Introduction A histological fixative is a chemical substance used to preserve biological tissues from decay, maintaining the structure of cells
Blood Vessels
Introduction Blood vessels deliver nutrients, oxygen and hormones to cells of the body and remove metabolic waste products and CO2
Preparation of 200 mL of 70% (v/v) ethyl alcohol
Introduction In histopathology, the use of alcohol solutions is crucial for preserving and fixing biological tissues. Alcohol, specifically ethyl alcohol,
Introduction to Histopathology
Introduction Introduction of Histopathology is the branch of pathology involving microscopic examination of biological tissues to observe the appearance of
Connective Tissue
General feature The term connective tissue (CT) is applied to a tissue that fills the interstices between more specialized elements.
Epithelial cells
Epithelial cells are specialised cells that form the epithelium, a continuous layer that covers body surfaces, lines cavities, and organs
Interference Microscope
Introduction An interference microscope is an advanced optical instrument that utilizes the principles of light interference to enhance image contrast
Determination of DLC
AIM: Determination of Differential Leukocyte Count (DLC) Principle The DLC is based on the microscopic examination of a blood smear
Connective and other mesenchymal tissues with their stains
Introduction Connective and other mesenchymal tissues with their stains is one of the four fundamental tissue types in the body,
Electron Microscope
Working Principle Electron microscopes use electron beams instead of light to magnify objects. Electrons have a much shorter wavelength than
Light Microscope
Working Principle The light microscope functions on the principle of magnification through visible light. When light is focused on the
Basic Histology I Yr, Medical Histopathology
Compound Microscope
Introduction A compound microscope is a sophisticated instrument that requires careful handling and maintenance to function effectively. By understanding its