The success of microtome sectioning depends on both technique and equipment. Below is an in-depth look at practical section cutting, common cutting faults, and how to address them to achieve Read More …
Practical section cutting
Author: Ms. Sangeeta Gourh
Microtome knives and knife sharpening
Introduction Microtome knives and knife sharpening are essential in laboratories, especially in histology and materials science, for preparing ultra-thin specimen sections suitable for microscopic examination. These knives must maintain sharp Read More …
Microtome
Microtomes are essential instruments in laboratories for cutting ultra-thin slices of specimens, particularly for microscopy. Different types are suited for various materials and methods. Here’s a breakdown of types, working Read More …
Decalcification
Introduction Decalcification is a critical preparatory step in histology, particularly for hard tissues such as bone and teeth, which contain large amounts of calcium that make them challenging to section. Read More …
Processing histological tissues for paraffin embedding
Processing histological tissues for paraffin embedding involves several key steps to ensure that the tissue is preserved, well-infiltrated with paraffin, and can be sectioned for microscopic analysis. Below is a Read More …