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        • SPECIALIZATION: MEDICAL BIOCHEMISTRY
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Author: Mr. Topesh Patle

Determination of Total Leukocyte Count

18/05/202518/05/2025 Mr. Topesh Patle

AIM: Determination of Total Leukocyte Count (TLC) Principle The glacial acetic acid lyses the red cells while the gentian violet slightly stains the nuclei of the leukocytes. The blood specimen Read More …

Hematology, Medical Hematology

Erythrocyte sedimentation rate

18/05/202507/09/2025 Mr. Topesh Patle

AIM: Determination of Erythrocyte sedimentation rate (ESR) Principle The ESR is based on the principle of gravity and the aggregation tendency of erythrocytes. In normal blood, erythrocytes have a negative Read More …

Hematology I Yr, Hematology, Medical Hematology, Pathology – IErythrocyte Sedimentation Rate (ESR), Factors Influencing ESR, Westergren method, Wintrobe method

Determination of Hemoglobin

18/05/202507/09/2025 Mr. Topesh Patle

AIM: Determination of Hemoglobin Principle of Sahli’s method When blood is added to 0.1 N hydrochloric acid, hemoglobin is converted to brown colored acidhematin. The resulting colour after dilution is Read More …

Hematology I Yr, Hematology, Medical Hematology, Pathology – I

Determination of Malaria Parasites

16/05/202507/09/2025 Mr. Topesh Patle

Determination of Malaria Parasites The thick blood smear concentrates Plasmodium parasites, making them easier to detect, while the thin smear allows detailed examination of the parasite’s morphology for species identification. Read More …

Hematology, Medical Hematology

Preparation of blood smear and staining method

27/04/202527/04/2025 Mr. Topesh Patle

Principle Blood smear preparation is used to examine blood cells under a microscope. It allows for the evaluation of the morphology of blood cells, identification of abnormalities, and determination of Read More …

Hematology

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