Introduction
- Accidents in a medical or biochemical laboratory can occur at any time due to chemicals, glassware, heat sources, or electrical equipment.
- Every laboratory must be prepared with first aid materials, trained personnel, and emergency procedures to handle injuries promptly and effectively.
- The goal of first aid is to reduce the severity of injury, prevent infection, and save life until professional medical help is available.

Common Causes of Laboratory Accidents
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Carelessness or lack of concentration while performing experiments.
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Improper handling of chemicals, acids, or glassware.
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Electrical short circuits or equipment malfunction.
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Fire or explosion due to flammable materials.
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Inhalation of toxic fumes or gases.
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Slipping on wet floors or broken glass.
Essential Laboratory First Aid Equipment
Every laboratory must have:
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A first aid box with antiseptic lotion, cotton, bandages, burn ointment, and pain relievers.
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Eye wash station and safety shower for chemical splashes.
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Fire extinguisher (CO₂ or dry chemical type).
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Blanket or fire-proof sheet for smothering flames on clothing.
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Emergency contact numbers displayed clearly.
Types of Laboratory Accidents and Their First Aid Measures
A. Chemical Burns (Acids and Alkalis)
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Immediately flush the affected area with large amounts of running water for at least 15–20 minutes.
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Remove contaminated clothing carefully.
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If the burn is due to acid, after washing, apply a mild alkaline solution (e.g., 1% sodium bicarbonate).
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If the burn is due to alkali, apply a mild acidic solution (e.g., 1% acetic acid or boric acid).
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Apply sterile dressing; do not apply ointments on deep burns.
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Seek medical attention immediately.
B. Thermal Burns (Heat or Fire)
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Cool the affected area with cold running water or ice packs for several minutes.
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Do not break blisters or remove burnt clothing stuck to the skin.
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Cover with a sterile non-adhesive dressing or clean cloth.
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For severe burns, call emergency services immediately.
C. Cuts and Wounds from Glassware
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Allow slight bleeding to flush out contaminants if the cut is minor.
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Wash with clean running water.
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Apply antiseptic solution (e.g., povidone-iodine).
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Cover with sterile gauze or bandage.
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If the wound is deep or glass remains embedded, do not attempt removal — seek medical help immediately.
D. Eye Injuries
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Chemical splash in eyes:
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Rinse eyes immediately at the eye wash station for at least 15 minutes, keeping eyelids open.
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Avoid rubbing eyes.
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Seek ophthalmologic treatment immediately.
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Foreign body in eye:
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Do not rub or attempt to remove it with fingers.
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Blink several times to help dislodge it or rinse gently with clean water.
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If pain persists, seek medical help.
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E. Inhalation of Toxic Fumes or Gases
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Immediately move the person to fresh air.
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Loosen tight clothing around neck and chest.
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If breathing is difficult, provide oxygen support if available.
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Keep the person warm and calm.
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Call for medical assistance immediately.
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Prevent further exposure by ventilating the lab and stopping the gas leak.
F. Ingestion of Chemicals:
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Do not induce vomiting unless directed by medical personnel.
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Rinse the mouth thoroughly with water.
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Identify the chemical ingested and provide its safety data sheet (SDS) to the healthcare provider.
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Take the person to the nearest hospital immediately.
G. Electrical Shock
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Turn off the power supply immediately before touching the victim.
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If unable to switch off, use a dry wooden stick or rubber-handled object to separate the victim from the electrical source.
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Check for breathing and pulse; if absent, start CPR (cardiopulmonary resuscitation).
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Treat visible burns as per burn protocol.
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Seek emergency medical help without delay.
H. Fire Accidents
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Raise the alarm and switch off all electrical devices and gas sources.
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For small fires, use a CO₂ or dry chemical fire extinguisher.
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For clothing fires, make the victim stop, drop, and roll, or wrap in a fire blanket to smother flames.
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Never use water on chemical or electrical fires.
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Evacuate the laboratory if the fire is uncontrollable.
I. Mercury Spill or Toxic Substance Spill
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Evacuate the area and prevent spread of vapors.
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Do not touch mercury with bare hands.
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Use a mercury spill kit or damp paper to collect droplets and store in a sealed container.
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Ventilate the room and call trained safety personnel for disposal.
J. Fainting or Shock
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Lay the person flat, with legs elevated slightly.
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Loosen tight clothing and ensure proper ventilation.
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Do not give food or drink until fully conscious.
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If the person does not regain consciousness, seek immediate medical care.
General Rules for Accident Prevention
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Always wear personal protective equipment (PPE): lab coat, gloves, goggles, and closed shoes.
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Avoid eating, drinking, or smoking in the lab.
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Label all chemicals properly and store them safely.
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Use fume hoods when working with volatile or toxic substances.
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Know the location of first aid kits, fire extinguishers, and emergency exits.
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Report all accidents and near-miss incidents to the lab supervisor immediately.
Emergency Reporting Procedure
Step 1 – Immediate Response:
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Ensure personal safety first — turn off electrical supply, remove sources of flame, and evacuate if needed.
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Provide first aid to the injured person using the laboratory’s first aid kit.
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If the accident is severe, immediately contact emergency medical services and inform the supervisor.
Step 2 – Notify Authorized Personnel:
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Report the incident to the laboratory supervisor or safety officer without delay.
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Provide clear information:
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Nature of the accident (chemical spill, fire, injury, electrical shock, etc.)
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Number of persons involved
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Type of injury or exposure
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Location of the accident (lab number, floor, building)
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Step 3 – Contact External Emergency Services:
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If internal control is not possible, contact fire, ambulance, or hospital services as per emergency protocol.
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Ensure clear access routes for emergency responders.
Step 4 – Secure the Area:
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Evacuate all non-essential personnel.
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Close laboratory doors and isolate the area if hazardous gases, fires, or spills are involved.
Step 5 – Follow-Up Actions:
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The injured person must be taken to the hospital or occupational health center for further care.
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All samples or equipment involved should be safely handled or sealed for investigation.
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Incident Reporting (Documentation)
Every laboratory must maintain an Incident or Accident Report Register that records all details for safety monitoring and prevention of recurrence.
Each report should include:
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Date and time of the incident
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Names of persons involved or injured
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Description of the accident (what happened, where, and how)
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Immediate actions taken (first aid, evacuation, etc.)
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Names of persons notified (supervisor, safety officer, etc.)
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Recommendations to prevent future occurrences
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Signature of the reporter and supervisor
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Display and Awareness
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Emergency contacts and procedures must be displayed prominently at:
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Laboratory entrance
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Near the first-aid box
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Chemical storage and fume hood areas
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Equipment rooms and corridors
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All staff and students should be trained during induction or safety orientation.