Determination of Total Protein
AIM: Determination of Total Protein Introduction Total Proteins are the most abundant compounds in serum. Amino acids are the building […]
MLT Practicals
Gram Staining
Principle Gram Staining is a differential staining technique developed by Hans Christian Gram in 1884. It works on the basis
AFB Stain
Principle The principle of AFB staining hinges on the unique properties of mycobacterial cell walls. These bacteria contain a high
Determination of Total Cholesterol
AIM: Determination of Total Cholesterol Introduction Total cholesterol is the total amount of cholesterol present in blood, including LDL, HDL,
Abnormal constituents of urine analysis
Introduction Urine analysis, also called urinalysis, is a commonly used diagnostic laboratory test. It is performed to evaluate the composition
Normal constituents of urine analysis
Introduction Urine is the final excretory product formed by the kidneys and reflects overall metabolic and renal function. Normal urine
Gross Examination of Histopathology
AIM: Gross Examination of Histopathological Specimens Principle The principle of gross examination of histopathology involves the visual and tactile assessment
ESR
AIM: Determination of ESR (Erythrocyte Sedimentation Rate) by the LANDAU Method Principles The Erythrocyte Sedimentation Rate (ESR) is a non-specific
Spore stain
Principle Spore staining relies on the ability of spores to resist conventional staining methods due to their tough, multi-layered coats.
Albert’s stain
Principle Albert’s stain works based on the unique ability of Corynebacterium species to accumulate metachromatic granules in their cytoplasm. These
Determination of Blood urea
AIM: Determination of Blood urea Non-enzymatic method Principle of Diacetyl monoxime method Under acidic conditions, when urea is heated with
Determination of Clotting Time
AIM: Determination of Clotting Time Capillary method Principle The principle behind the capillary method is that blood, when exposed to
Determination of Bleeding Time
AIM: Determination of Bleeding Time Duke Method Principle: The Duke method is a simple test to measure the time taken
Determination of blood sugar
AIM: Determination of blood sugar Introduction Blood sugar, also called blood glucose, is the amount of glucose (a type of
Determination of Reticulocyte Count
AIM: Determination of Reticulocyte Count Principle The supravital staining method is used for reticulocyte count. Blood is mixed with the
Determination of Platelet Count
AIM: Determination of Platelet Count (Thrombocyte) Principle The total platelet count is a blood test used to measure the number
Determination of DLC
AIM: Determination of Differential Leukocyte Count (DLC) Principle The DLC is based on the microscopic examination of a blood smear
Determination of PCV
AIM: Determination of PCV (Packed Cell Volume), also known as hematocrit (HCT) Microhematocrit Method Principle The principle of the microhematocrit
Determination of Total Leukocyte Count
AIM: Determination of Total Leukocyte Count (TLC) Principle The glacial acetic acid lyses the red cells while the gentian violet
Erythrocyte sedimentation rate
AIM: Determination of Erythrocyte sedimentation rate (ESR) Principle The ESR is based on the principle of gravity and the aggregation
Determination of Hemoglobin
AIM: Determination of Hemoglobin Principle of Sahli’s method When blood is added to 0.1 N hydrochloric acid, hemoglobin is converted
Determination of Malaria Parasites
Determination of Malaria Parasites The thick blood smear concentrates Plasmodium parasites, making them easier to detect, while the thin smear
Preparation of blood smear and staining method
Principle Blood smear preparation is used to examine blood cells under a microscope. It allows for the evaluation of the
Determination of abnormal hemoglobin by various methods
Determination of abnormal hemoglobin by various methods Electrophoresis is based on the principle of charge-to-mass ratio of molecules. Hemoglobin molecules
Routine Staining and Microscopy of Stool for Ova and Cysts
Principle Staining techniques is to improve the visibility of intestinal parasites (ova, cysts, trophozoites) within stool samples through selective staining
Microbiology
Methods for Determining Motility of Bacteria
Motility of Bacteria Motility refers to the ability of bacteria to move actively and independently. It is an important characteristic
Electron Microscope
Working Principle Electron microscopes use electron beams instead of light to magnify objects. Electrons have a much shorter wavelength than
Light Microscope
Working Principle The light microscope functions on the principle of magnification through visible light. When light is focused on the
Biochemistry III Yr, Biochemistry, Biochemistry - 2nd Yr
SGPT
Introduction Serum Glutamate Pyruvate Transaminase (SGPT), also known as Alanine Aminotransferase (ALT), is a liver enzyme primarily involved in amino
Biochemistry III Yr, Biochemistry, Biochemistry - 2nd Yr
SGOT
Introduction SGOT is an enzyme that catalyzes the reversible transfer of an amino group between aspartate and α-ketoglutarate to form