Simplifying Allied Health Learning.
AIM: Determination of Total Cholesterol Principle of an enzymatic method Cholesterol ester + H2O ————>Cholesterol esterase —————> Cholesterol + Fatty acids Cholesterol + O2 —————->Cholesterol oxidase —————–> Cholesterol-4-en-3-one + Read More …
AIM: Determination of PCV (Packed Cell Volume), also known as hematocrit (HCT) Microhematocrit Method Principle The principle of the microhematocrit method is based on the separation of blood components by Read More …
AIM: Determination of Total Leukocyte Count (TLC) Principle The glacial acetic acid lyses the red cells while the gentian violet slightly stains the nuclei of the leukocytes. The blood specimen Read More …
AIM: Determination of Erythrocyte sedimentation rate (ESR) Principle The ESR is based on the principle of gravity and the aggregation tendency of erythrocytes. In normal blood, erythrocytes have a negative Read More …
AIM: Determination of Hemoglobin Principle of Sahli’s method When blood is added to 0.1 N hydrochloric acid, hemoglobin is converted to brown colored acidhematin. The resulting colour after dilution is Read More …